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This grant aims to fund a project focusing on the genetic identification of Yosemite toads and western toads, as well as their intergrades. The research will use RADseq next-generation sequencing to discover single nucleotide polymorphisms for species identification. By creating species-diagnostic markers, the project aims to assist in various ecological studies including species delimitation, interspecific hybridization, and genetic diversity. The grant is a single source opportunity and not open for application. Closing date for this grant is July 10, 2012.
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Opportunity ID: 179913
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General Information
| Document Type:: | Grants Notice |
| Funding Opportunity Number:: | F12AS00259 |
| Funding Opportunity Title:: | Genetic Identification of Western and Yosemite Toads and Intergrades |
| Opportunity Category:: | Discretionary |
| Opportunity Category Explanation:: | |
| Funding Instrument Type:: | |
| Category of Funding Activity:: | Environment |
| Category Explanation:: | |
| Expected Number of Awards:: | 1 |
| Assistance Listings Number(s):: | 15.650 — Research Grants (Generic) |
| Cost Sharing or Matching Requirement:: | No |
| Version:: | Synopsis 1 |
| Posted Date:: | Jun 26, 2012 |
| Last Updated Date:: | |
| Original Closing Date for Applications:: | Jul 10, 2012 |
| Current Closing Date for Applications:: | Jul 10, 2012 |
| Archive Date:: | |
| Estimated Total Program Funding:: | $ 94,322 |
| Award Ceiling:: | $94,322 |
| Award Floor:: | $94,322 |
Eligibility
| Eligible Applicants:: | Public and State controlled institutions of higher education |
| Additional Information on Eligibility:: |
Additional Information
| Agency Name:: | Fish and Wildlife Service |
| Description:: | This funding announcement is a Notice of Intent to Award. PLEASE DO NOT APPLY FOR THIS OPPORTUNITY. Federal regulations require that all opportunities be posted. This is intended to comply with those regulations and is a single source opportunity Yosemite toad (Anaxyrus canorus) populations have declined substantially in recent decades, though the reasons for declines are unclear; disease (chytridiomycosis), contaminants (pesticide drift), and grazing have been implicated as potential causes. The more common, conspecific western toad (Anaxyrus boreas) generally inhabits lower elevation habitats, however, overlaps with A. canorus in many localities. Given the difficulty in identifying larval, juvenile, and sometimes even adult individuals to species in the field, simple molecular genetic assay for species identification would aid in identification. We propose a RADseq next-generation sequencing approach to discover thousands of single nucleotide polymorphisms that can be used to first build a clear set of phylogenetic relationships among individuals sampled from throughout a representative geographic sample of both species. The same data set may then be mined for species-diagnostic markers that form a reliable and rapid means of identifying species. Such markers could be employed in studies such as species delimitation, interspecific hybridization, parentage/relatedness, genetic diversity, and variety of other ecological questions. |
| Link to Additional Information:: | |
| Grantor Contact Information:: | If you have difficulty accessing the full announcement electronically, please contact: |
Version History
| Version | Modification Description | Updated Date |
|---|---|---|
| Synopsis 1 |
Package Status
Package No: 1
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